论文投稿_医学论文投稿_核心期刊,职称论文投稿发表_论文部落

　　摘要目的：研究生脉饮对病毒性心肌炎心肌细胞凋亡作用的影响。方法：用柯萨奇B病毒建立感染的心肌细胞模型，在感染后24小时将细胞分为正常对照组、病理模型组、生脉饮组和辅酶Q10组。4组分别在不同时间点观察细胞形态，并采用TUNEL法进行细胞凋亡的检测。结果：在48小时点开始检测，正常对照组无细胞凋亡，生脉饮组及辅酶Q10组均有凋亡，生脉饮组凋亡率低于辅酶Q10组（P<0.05），论文范文病理模型组随着时间的推移，凋亡率增加，72小时点明显高于48小时点（P<0.01），与药物干预组比较差异具有统计学意义（P<0.05）。结论：生脉饮能够有效抑制病毒性心肌炎心肌细胞的凋亡，且应该早期干预。 

　　关键词生脉饮；病毒性心肌炎；细胞凋亡 

　　Study on the apoptosis effect of pulse-activating decoction compound preparation in viral myocarditis 

　　Xiong Lihui1，Sun Dashan2 

　　Basic Medical Colleg，Changchun Traditional Chinese Medicine University，1301171 

　　2012 Grade Traditional Chinese medicine Professional Experimental Class，Guangzhou Traditional Chinese Medicine University（Guangzhou City，Guangdong），1301172 

　　AbstractObjective：To study the effect of pulse-activating decoction compound preparation in myocardial apoptosis effect of viral myocarditis.Methods：Using coxsackie B virus established infectious myocardial cell model.In 24 hours after infection，the cells were divided into four groups：the normal control group，the pathological model group，the pulse-activating decoction group and the coenzyme Q10 group.The cellular morphology of four groups of cells were observed at different time points.The apoptosis was detected by TUNEL method.Results：Begin testing at 48 hours，the normal control group had no apoptosis；the pulse-activating decoction group and the coenzyme Q10 group all had apoptosis；the apoptosis rate of the pulse-activating decoction group was lower than that of the coenzyme Q10 group（P<0.05）.In the pathological model group，the apoptosis rate was increased as time goes on.72 hours point was obviously higher than 48 hours point（P<0.01）.There had statistically significant compared with the drug intervention group （P<0.05）.Conclusion：Pulse-activating decoction can effectively inhibit the myocardial cell apoptosis of viral myocarditis，and it should be early intervented. 

　　Key wordsPulse-activating decoction；Viral myocarditis；Apoptosis 

　　病毒性心肌炎（viral myocarditis，VM）是指病毒感染引起的心肌局限性或弥漫性的急慢性炎性病变，多由柯萨奇病毒引起。在柯萨奇病毒导致VM的病理机制研究中，越来越多的证据表明，柯萨奇病毒导致病毒性心肌炎心肌细胞凋亡，从而出现心肌坏死病变[1]。中医学认为VM属于“心悸”“怔忡”范畴，病机中多存在气阴两虚，治疗上以益气养阴为主，多采用生脉饮进行加减治疗，其组成为人参、麦冬、五味子。生脉饮治疗VM临床疗效确切，但其干预机制尚不明确，且临床上生脉饮是否应该早期应用一直是临床争议的问题。为此，本实验拟观察生脉饮对VM心肌细胞凋亡的影响，力求明确生脉饮的作用机制以及最佳时间，为临床准确使用提供参考。 

　　资料与方法 

　　材料：①实验动物：新生SD（Sprague-Dawlay）大鼠，1～3日龄，由吉林大学基础医学院动物实验中心提供。②病毒株：柯萨奇B病毒试剂盒（Coxsackie Virus B3）。③实验药物：生脉饮注射液，辅酶Q10注射液，胰蛋白酶（1：250），Sigma；DMEM（dulbec’s modified eagle）培养基，GIBCO；胎牛血清，GIBCO；牛血清白蛋白，GIBCO。④溶液配制：缓冲液：Na2HPO4·2H2O 0.06 g，KH2PO4 0.06 g，MgSO4·7H2O 0.20 g，葡萄糖1.00 g，NaCl 8.00 g，KCl 4 g，CaCl2 0.14 g，三蒸水1 000 mL。DMEM培养液：20%胎牛血清、100 U/mL青霉素、100 mg/mL链霉素及谷氨酰胺。细胞消化液：由Ⅰ型胶原酶20 mg、胰蛋白酶18 mg、牛血清白蛋白10 mg、加Eagle培养基10 mL。

　　摘要目的：研究生脉饮对病毒性心肌炎心肌细胞凋亡作用的影响。方法：用柯萨奇B病毒建立感染的心肌细胞模型，在感染后24小时将细胞分为正常对照组、病理模型组、生脉饮组和辅酶Q10组。4组分别在不同时间点观察细胞形态，并采用TUNEL法进行细胞凋亡的检测。结果：在48小时点开始检测，正常对照组无细胞凋亡，生脉饮组及辅酶Q10组均有凋亡，生脉饮组凋亡率低于辅酶Q10组（P<0.05），病理模型组随着时间的推移，凋亡率增加，72小时点明显高于48小时点（P<0.01），与药物干预组比较差异具有统计学意义（P<0.05）。结论：生脉饮能够有效抑制病毒性心肌炎心肌细胞的凋亡，且应该早期干预。 

　　关键词生脉饮；病毒性心肌炎；细胞凋亡 

　　Study on the apoptosis effect of pulse-activating decoction compound preparation in viral myocarditis 

　　Xiong Lihui1，Sun Dashan2 

　　Basic Medical Colleg，Changchun Traditional Chinese Medicine University，1301171 

　　2012 Grade Traditional Chinese medicine Professional Experimental Class，Guangzhou Traditional Chinese Medicine University（Guangzhou City，Guangdong），1301172 

　　AbstractObjective：To study the effect of pulse-activating decoction compound preparation in myocardial apoptosis effect of viral myocarditis.Methods：Using coxsackie B virus established infectious myocardial cell model.In 24 hours after infection，the cells were divided into four groups：the normal control group，the pathological model group，the pulse-activating decoction group and the coenzyme Q10 group.The cellular morphology of four groups of cells were observed at different time points.The apoptosis was detected by TUNEL method.Results：Begin testing at 48 hours，the normal control group had no apoptosis；the pulse-activating decoction group and the coenzyme Q10 group all had apoptosis；the apoptosis rate of the pulse-activating decoction group was lower than that of the coenzyme Q10 group（P<0.05）.In the pathological model group，the apoptosis rate was increased as time goes on.72 hours point was obviously higher than 48 hours point（P<0.01）.There had statistically significant compared with the drug intervention group （P<0.05）.Conclusion：Pulse-activating decoction can effectively inhibit the myocardial cell apoptosis of viral myocarditis，and it should be early intervented. 

　　Key wordsPulse-activating decoction；Viral myocarditis；Apoptosis